Essay on Concentrations of Bile Salts on Lipase Enzyme

4448 Words Feb 13th, 2007 18 Pages
The aim of this investigation is to explore the effect of different concentrations of bile salts on the time taken for the lipase enzyme to break down fat.

Bile is a brownish bitter alkaline fluid produced by the liver and made by the hepatocytes from water, bile salts, bile pigments cholesterol and phospholipids and stored in the gall bladder. Bile is directly connected with digestion. It is released sporadically into the small intestine (duodenum) which is part of the gut in order to help digestion. Bile contains chemicals that break down/emulsify fats by dispersing fat globules into small droplets, therefore increasing the surface area, in turn speeding up the reaction. When fats get to the small intestine they
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There are two types of inhibitors; competitive, and non-competitive.
Competitive Inhibitors are a similar shape to the substrate, therefore can compete with the substrate to block the active site, leaving the substrate no way to bind with the enzyme and react.
Non-Competitive Inhibitors bind with a different part of the enzyme causing it to disfigure, leaving the substrate no way to bind as the active site has changed. They do not compete with the substrate for the active site.

The rate of reaction depends on how often and how hard the reacting particles collide with each other. Particles have to collide in order to react, and they have to collide hard enough as well. This is called the collision theory. When the temperature is increased the particles all move quicker. If they are moving quicker then they are going to have more collisions. Higher temperature also increases the energy of the collisions, because it makes all the particles move faster. Increasing the temperature causes faster collisions. Reactions only happen if the particles collide with enough energy. At a higher temperature there will be more particles colliding with enough energy to make the reaction happen. This initial energy is known as the activation energy, and it is needed to break the initial bonds.

Low Concentration –
Particles in a low concentration are more spread out because there are fewer particles, therefore fewer

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