How Eukaryotic Cells Contain Several Membrane Bound Organelles

1659 Words Feb 29th, 2016 null Page
Question and Hypothesis
Experiment I
Eukaryotic cells contain several membrane-bound organelles. Plant cells are a specific type of eukaryote that are both aerobic and photosynthetic. Because of this, they contain mitochondria that serve a vital role in aerobic respiration that follows photosynthesis. The enzyme succinate dehydrogenase (SDH) is an enzyme associated with the Citric Acid Cycle that takes place in the mitochondrial matrix. SDH serves as a catalyst for a reaction in which succinate and FAD become fumarate and FADH2. The SDH-FADH2 complex then reduces Ubiquinone in the electron transport chain. SDH can be indirectly measured using the artificial electron acceptor DCIP that is blue in its oxidized state and colorless in its reduced state. In other words, when the SDH-FADH2 complex reduces DCIP by transferring the electrons from the hydrogen in FADH2, the solution becomes colorless. However, in order for the electrons to go to the DCIP and not Ubiquinone as they naturally do, sodium azide must be used to stop electrons from flowing through the electron transport chain. Therefore, by fractionating bean sprouts using centrifugation, it can be determined which cell fraction contains the most mitochondria. This is the big question that experiment I is aimed to answer. (Leicht and McAllister, 2015).
Because centrifugation separations homogenate based on size and density of its components, figuring out which fraction has the highest abundance of mitochondria is based…

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