Increasing Sucrose Concentration Deceases Change in Mass of Potato Tuber Segments; Relative Osmotic Concentration Determined by Percent Change in Mass

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The relative osmotic concentration was determined by measuring the percent change in mass of the potato tissues. Change in mass was measured of six solutions, each containing different levels of concentration (0, 0.1, 0.2, 0.3, 0.4, and 0.5). The percent change in mass decreased as sucrose concentration increased, therefore, relative osmotic concentration also decreased as sucrose concentration increased. However, the osmotic concentration of 0.2 M sucrose solution was relatively greater than that of 0.1 M sucrose solution. In sucrose concentration 0.5 M, the osmotic concentration decreased slightly from that of 0.4, but significantly from those of all other sucrose concentrations. The osmotic concentrations were greater than
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The null hypothesis that as the sucrose solution increases, there will be no different in the change of mass was rejected. Our alternative hypothesis that as the sucrose solution increases the change in mass will increase was also rejected.

Osmosis occurred at each level of sucrose concentration measured. It was observed that osmotic concentration decreased as sucrose concentration increased. This may be because water moved from the less concentrated potato tuber cells to the greater concentrated sucrose solution (Kargol, 1996). Factors which influence the movement of materials across a cell membrane include size, charge, hydrophobic/hydrophilic, and concentration. Since the process of osmosis is with a selectively permeable membrane, only water is able to move across the membrane. Therefore, the only factor influencing the process of osmosis is concentration.

The potato tuber segments incubated in the 0.2 M sucrose concentration had an unfitting change of mass when compared to the trends of the results. This could not be explained, and was declared as human error. One explanation for this result is that the wrong sucrose concentration was used; instead of the 0.2 M concentration being used, it was the 0 M concentration. This would make sense because the results of the two are similar. A suggestion for this experiment in the future would be to

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