Micro-extraction of urine and/or stomach contents and analysis by TLC
Thin Layer Chromatography (TLC) is one of the oldest chromatographic techniques used for the identification of compounds and for determination of the presence of trace impurities.
The aim of the experiment was to determine two unknown samples (X and Y) by comparing results to the known standard drugs, of which there was five, using a specific TLC method.
The same technique was used for the determination of the unknown; however, the solution used for each method was slightly different. Furthermore, the mobile phase in each method was of different concentration with the addition of ethylacetate in Solvent system A.
The distance moved by the spot …show more content…
The TLC place with solution X on was placed in Beaker A, which contained Solvent System A and the other TLC placed was placed in Beaker B, which contained Solvent System B. Both beakers were then covered with foil and allowed to develop for 15 minutes.
Both TLC plates were removed from the beakers and the solvent front was marked with a light pencil. A hairdryer was used to speed up the process of drying. The TLC plates were then placed under a UV lamp to detect any visible spots and with a pencil were circled. The color of the spots was also identified while under the UV lamp.
Results and Discussion
Development time – 15 minutes
Distance migrated my solvent front - 61mm
Distance moved by spot (mm)
RF= Distance moved by spot Distance migrated by solvent