Microbiology 150 Lab 3-Selective vs. Differential Media Essay

2927 Words Jan 22nd, 2013 12 Pages
Online BIO 150 Introductory Microbiology #3 Lab Report

NAME __ Lab Group 2_____

Answer the following questions as you work your way through the lab material typing in your answers. Then submit your finished lab report as a Microsoft Word document. This lab report is worth 100 points towards your final lab grade. Each Q is worth 2 points unless otherwise noted. Also, per the Honor Code, this work must be your own. This is due Mon. 10/8 at 11:59 PM. The theme of this lab is the identification of unknown bacteria and viruses in a lab.

Selective vs. Differential Media

Selective vs. Differential Media
Use the following website to help you answer Q 1 and 2
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The next step in identification is morphology.

8. The remaining flow chart pages will show you how dichotomous keys are used in bacterial identification. Scroll down to page 7 and look at the Family Enterobacteriaceae which is comprised of Gram negative rods. (4 pts.)
a. What is the first test that may be performed to start differentiating among the individual species?
The first test that may be performed to start differentiating among the individual species is Lactose Fermentation.

b. Use of a dichotomous key allows you to perform the next test needed to identify your mystery microbe based on the results of the test you just performed, i.e. were the results positive + or negative -. Using the flowchart, what would be the microbe which has these test results: Lactose positive (+), Indole positive (+) and Citrate negative (-)?
The microbe would be Escherichia Coli.

ATLAS SECTION 2: SELECTIVE MEDIA.
I would like for you to read over the different types of selective media and then answer the following questions. Remember that selective media promote the growth of some bacteria while actively discouraging the growth of others.

9. For what is Chocolate II agar used? ( 2 pts.) Chocolate II agar is used for isolation and cultivation of Neisseria and Haemophilus.

10. Based on the information in the Principle section, Phenylethyl Alcohol Agar will select for the

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