The Effect of Temperature on the Enzyme Catalase Essay

3514 Words Apr 18th, 2013 15 Pages
The Effect of Temperature on the Enzyme Catalase Stephen Francis
Biology 183
Abstract
This experiment was performed to determine the resultant effect of temperature change on the reaction between the enzyme catalase and hydrogen peroxide. This experiment was performed by measuring and comparing the amount of oxygen bubbles produced and the absorbance of the catalase and hydrogen peroxide solution over time at room temperature, 2°C, 50°C, and 60°C. The overall result of this experiment proved that this reaction works best at room temperature, slows down when the temperature is lowered, and does not take place when the temperature gets too high due to the enzyme denaturing at these higher temperatures. The hypothesis that was
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Next the temperatures of the test tubes in the ice bucket were measured and recorded. After this, the test tubes were mixed based on their corresponding letters and the reactions were observed and results recorded and compared to each other, and the results of the first part of this experiment.
Procedure B: Absorbance Levels This part of the experiment measured the change in absorbance at 470nm for the reaction between catalase and hydrogen peroxide. First two test tubes were labeled “Blank” and “1”. For the blank test tube, 6mL of deionized water, 100µL of the indicator guaiacol, and .1% H2O2 were mixed together using pipets for the water and micropipettes for the guaiacol and hydrogen peroxide. Then a piece of aluminum foil was placed on top of the test tube to cover it and the test tube was inverted a few times to ensure that the solution was mixed well. A curvette was then filled with the blank solution an inserted into the spectrometer and read as the blank. After this measurement was taken, the spectrometer was set to subtract the blank from the “1” solution. The other test tube was then filled with 1mL of catalase, 5mL of deionized water, 100µL of guaiacol, and .1% H2O2. It was important to make sure the substrate is mixed in last to ensure the experiment works correctly. Then the solution was inverted using the aluminum foil as with the blank, and placed in a curvette and into the spectrometer. The absorbance

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