Twin-Pulse Facilitation Measured as Twitch Ratios in the Frog Sciatic Nerve/Gastrocnemius Muscle Preparation
Richard S. Manalis and Jonathan L. Miller— Department of Biology, Goshen College, Goshen, Indiana
Learning & Memory are related to synaptic efficacy which the present study brings to the undergraduate physiology laboratory. TWIN-PULSE FACILITATION has been widely used to study one aspect of short-term synaptic plasticity at a wide variety of synapses using electrophysiological methods. Here we show that measurements of muscle twitches from the easily prepared sciatic nerve/gastrocnemius preparation of the frog can allow one to perform experiments involving TWIN-PULSE FACILITATION. The advantages of measuring muscle twitches …show more content…
CALCULATION OF THE ! T/C RATIO! Using LabScribe software and with reference to Fig. 2, one cursor is placed on b (baseline) and the other one is placed on c (C peak); the voltage difference between the cursors is taken as the amplitude of the C twitch at its PEAK. Then, without moving the cursor on point b, the second cursor is placed on t (T peak); the voltage difference between the cursors is taken as the amplitude of the T twitch at its PEAK.