Worm Lab Report Essay

1095 Words Jan 29th, 2013 5 Pages
Title: The Effect of Nicotine on the Heart Rate of the Lumbriculus Variegatus
Background- This lab is being done to demonstrate the effects of stimulants on the circulatory system of blackworms. In this lab, the stimulant we have chosen to use is Nicotine. A stimulant is an agent that causes increased activity, especially in the nervous or cardiovascular systems. In this lab, we will put blackworms into the Nicotine to look at the differences in pulse rates when compared to a control. The proper name for blackworms is Lumbriculus Variegatus, they are freshwater worms most commonly found in North America and Europe. The blood in blackworms circulates from the dorsal blood vessel toward the head through a series of
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Take the worm and put it into a separate pipette and then another capillary tube and measure the pulse rate for one minute. Take the worm out and put it into the “rehab” petri dish, a place for the worms to get their heart rates back to normal before joining all of the worms. Continue these steps and record the pulse rate once in the nicotine for each of the worms. Make sure to calculate both the mean and median from both the control and stimulant trials.
-The dependent variable is the pulse rate of the blackworms in pulse/min. The independent variable is the nicotine added which is 2.5mL with 1mL H2O. The same 15 worms were used in both trials ( each worm was tested once for each), the same Nicotine was used for all stimulant trials, worms were in the Nicotine for the same amount of time, the pulse rate was recorded at the same time for each worm, same capillary tubes were used, same pipette was used, and worms were put into the same petri dishes. Two trials were done with the Nicotine at 2.5 mL and nine trials were done with 2.5 mL Nicotine along with 1 mL H2O. We had to dilute the solution because every worm in the 2.5mL Nicotine solution died. Spring water served as the positive control since it serves as the worm’s everyday habitat. All data will be compared to this control.

Table 1 – Control Group for Heart Rate Worm # | Pulse/Min | 1 | 9 | 2 | 8 | 3 | 13 | 4 | 13 | 5 | 8 | 6 | 8 | 7 | 10 | 8 | 7

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