Research on Mathematical Calculations of Bonds Betwen Amino Acid Residues and CO2 Molecules

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Cundari et al presented a powerful computational prediction system based on mathematical calculations of bonds between amino acid residues and CO2 molecule (Cundari et al. 2009). In their study, binding energies for CO2 and amino acid residues of Rubisco active site differ between different enzyme species that belongs to different organisms. It has been suggested that most of the hydrogen bonding in α-helices goes toward stabilizing the tightly coiled helix in loop 6 of Rubisco, whereas in contrast the edge of a β-sheet is open to hydrogen bonding, the binding energies are shown in table 1 (Cundari et al. 2009). Cundari also compared the residues binding energy to CO2 with other molecules such as methan, phenol, and benzen. Interestingly, …show more content…
The first discovery of RCA enzyme was in the mutant Arabidopsis rca in early 1980s (Portis and Salvucci 2002) that came from screening for Photorespiration defects under high/low CO2 growth conditions. Since the discovery, RCA was studied in detail through mutagenesis and screening studies until it was known to be responsible for the removal of the tightly bound sugar phosphate from Rubisco active site through ATP hydrolysis (Portis 2003). Salvucci et al reported the characteristics of RCA heat sensitivity and it’s effect on photosynthesis and Rubisco activity (Salvucci et al. 2001). Their results indicated that RCA is easily denatured during heat stress, causing protein aggregates in vitro, in the presence or absence of ATP, while in vivo, Rubisco was deactivated and chloroplast proteins showed a degraded form of RCA (Salvucci et al. 2001). Mueller-Cajar and Whitney established huge mutant libraries looking for an RCA mutant that can rescue rca Arabidopsis. They found three mutants coding for (T274R), (P168L, V257I, and L301R), and (P168L, V257I, T247R, and L301R) (Mueller-Caja and Whitney 2008), which were transformed into the Δrca Arabidopsis, their results showed how transformed plants, with any of the three mutant RCA forms, were able assimilate CO2, grow and give better seed yield when grown at temperatures above normal. Furthermore, Kurek et al went in details for this phenomenon of RCA heat sensitivity and generated mutant libraries

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