Storage Duration of Mother Scales' Effects on Soluble Carbohydrates and Amylases in Lily Scale Bulblets

1952 Words 8 Pages
1. Introduction
The scaling is a technique for propagation of Lilium (Gray, 1974; Marinangeli and Curvetto, 1997; Marinangeli et al., 2003). The mother bulbs are scaled and stored in a favorable substrate. Scale bulblets develop on the sides of the individual mother scale (Gray, 1974). This method was studied and reported earlier (Matsuo, 1972; Matsuo and Van Tuyl, 1984; Matsuo et al., 1989; Cabrera, 2002). Addai (2010) found the sucrose as an essential and major carbohydrate in Lilium bulb, because breaking and metabolism of this sugar are important to facilitate the growth of storage organ, inflorescence and others. However, Addai (2010) noticed that starch is a major carbohydrate and as an energy source for bulbous plants such as lily
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Bulbs, corms, tubers and other storage organs of plants contain the hydrolactic enzymes of starch, i.e. α- and β- amylases (EC 3.2.1.1 and EC 3.2.1.2, respectively). β- Amylase is an exo-enzyme which breaks α-1,4-glucoside bands from the non-reducing end of starch, and produces maltose and β- limit dextrin molecules. However, α- amylase is an endo-amylase, which breaks non-terminal glucosidal bands, and produces glucose, maltose and branched oligosaccharides (Addai, 2010).
The content of carbohydrate and the activity of amylases affect quality of sprouting scale bulblets after vernalization. The objective of this study was evaluating how the effect of storage duration on the quality (metabolism of soluble carbohydrate and the activities of amylases) in ʻArabian Redʼ.
2. Materials and methods
2.1. Plant material
Middle Scales on basal plate oriental hybrid lily ʻArabian Redʼ bulbs (18 - 20 cm in circumference, Van den Bos, Netherlands) with the same weight (2 - 2.5 g) were scaled. Scales were dipped for 15 min in a fungicide solution containing 1% (w / v) benomyl. After air drying, 10 scales were placed in polyethylene bags with some pores, containing ≈ 1 liter of moist coco peat and perlite (1 : 1, v : v; 85-90% moist). The bags were placed at 25 ˚C in the growth chamber for 3 or 5 months (Marinangeli and Curvetto, 1997). After storage duration (3 or 5 months) and vernalizing scale

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